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In five of these streams, we also sampled repeatedly during autumn to test if stereospecific information into quantitative data by means of a novel strategy; typical features of tension wood in hybrid aspen SO NEW PHYTOLOGIST LA English DT soil communities and potentially interact with plant-microbial feedbacks.
Bi- and tri-parental exogenous plasmid isolations from eight bulk soils before and after mercury addition Potential plasmid recipients can stretch across diverse microbial groups , and although transconjugants within sink species may be transient (due to segregation or purifying selection) , their continual replenishment by conjugation from the source means that microbial community richness may be more robust to occasional bouts of selection for plasmid-borne genes. Moreover, conjugation via plasmid transfer is postulated to be prevalent in complex microbial communities (e.g., the gut and soil microbiomes) due to the high local density, diversity, and abundance of strains/species along with mobile genetic elements (Ogilvie et al, 2012). In particular, associations exist between resistance genes and bacterial communities via vertical and horizontal transfers, and as mcr-1 and mcr-3 plasmids are highly transferable (conjugation rates are as high as 10 −4, unpublished data), there is the potential risk of mcr-1 and mcr-3 genes being rapidly disseminated throughout bacterial communities. 2019-10-09 · Cover crop suppression with glyphosate-based herbicides (GBHs) represents a common agricultural practice. The objective of this study was to compare rhizospheric microbial communities of A. sativa plants treated with a GBH relative to the mechanical suppression (mowing) in order to assess their differences and the potential implications for soil processes.
2014, 5 (December), Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne, Barth F Smets; Affiliations Uli eKlümper Technical University of Denmark Ariadni eDroumpali Technical University of Denmark Arnaud eDechesne By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Gelder, Grahn, Gregory, Grohmann, Götz, Haase, Heuer, Heuer, Heuer, Hill, Hirt, Honda, Klümper 2010-05-28 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Published in: Frontiers in Microbiology, December 2014 DOI: 10.3389/fmicb.2014.00730: Pubmed ID: 25566238. Authors: Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets Abstract: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper; Ariadni Droumpali; Arnaud Dechesne; Barth F. Smets; Frontiers in Microbiology. Published on 21 Dec 2014. 8,751 views XX downloads; XX citations; View Antibiotic resistance gene transfer mediated by plasmids is a matter of concern for public health, but permissive environments supporting plasmid dissemination are still quite difficult to identify.
Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets. Department of Environmental Engineering; Research output: Contribution to journal › Journal article › Research › peer-review.
Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper Novel Assay To Assess Permissiveness of a Soil Microbial Community toward Receipt of Mobile Genetic Elements
REP-PCR analysis of selected transconjugants that received plasmid pIE723 in the wheat rhizosphere. Lanes: 0, molecular size ladder; 1, Agrobacterium 1998-12-01 2020-10-26 The ability to introduce individual molecules of plasmid DNA into cells by transformation has been of central importance to the recent rapid advancement of plasmid biology and to the development of DNA cloning methods. Molecular genetic manipulation of bacteria requires the development of plasmid-me … A physical map of a mobilizable plasmid pMC2, showing the genetic organization and structure of the plasmid. The different colours and patterns represent all the predicted genes (solid dark shading) and regions encoding putative functions such as replication and maintenance (boxed), antibiotic resistance (cross‐hatched), heavy metal resistance (vertical lines), transposons and insertion 2006-01-01 2019-10-09 These range from the very rapid (infectious) spread of traits associated with plasmids like RP4 through a bacterial community, which can eventually occupy up to 100% of potential recipient cells in just a few hours under the appropriate conditions, to the apparent reluctance of structured microbial communities to accept exogenous DNA or active MGEs.
Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB)
We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. 149 bacterial communities toward conjugal plasmids by Smets, B. F. Novel assay to measure the plasmid 156 mobilizing potential of mixed microbial communities. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets.
2016-09-23 · By using plasmid-encoded TEM β-lactamase mediated ampicillin resistances as a proof of principle system, we (1) developed a fluorescence in situ hybridization-test (FISH) capable to detect the respective mRNAs, (2) implemented an immunofluorescence test to identify the corresponding proteins and (3) compared these two microscopic tests with an established colorimetric nitrocefin assay to
2019-08-28 · 39 plasmids, members within complex microbial communities might have a hitherto unrecognized 40 potential to maintain plasmids for long-term community-wide access. 41 available under aCC-BY-NC-ND 4.0 International license. not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. 2016-09-23 · However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures.
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Published on 21 Dec 2014. 8,751 views XX downloads; XX citations; View
Antibiotic resistance gene transfer mediated by plasmids is a matter of concern for public health, but permissive environments supporting plasmid dissemination are still quite difficult to identify. Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1β plasmid pB10 in natural microbial communities maintained in microcosms. 2017-07-12
2014-02-10
Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.
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It has been demonstrated that the lack of novel effective antibiotics and rapid spread of Notably, plasmid-mediated horizontal transfer of antibiotic resistance genes Generally, this process includes two steps: (1) DNA is mobilize
This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets. Department of Environmental Engineering; Research output: Contribution to journal › Journal article › Research › peer-review. 149 bacterial communities toward conjugal plasmids by quantification and isolation of Smets, B. F. Novel assay to measure the plasmid 156 mobilizing potential of mixed microbial communities.